Step by Step Process of IVF

Step by Step Process of IVF

Many people suffer from infertility across the globe. The latest technological and medical advancements have made it possible to provide such people with the boon of having a baby.

One of the most prominent procedures available today is the In Vitro Fertilization (IVF) procedure. IVF is a series of procedures used to help with fertility or prevent genetic problems and assist with the conception of a child.

The process involves fertilizing the egg with the sperm in an incubator outside the body, then transferring the best embryo back into the woman’s uterus. The whole process, up to the embryo transfer stage, usually takes three to four weeks.

If after an initial consultation (testing and diagnosis) we as your specialists determine that IVF is the best plan for you, then below are the normal steps that the treatment will follow.

Step 1: Starting treatment

During a regular menstrual cycle, hormones from the pituitary gland – Luteinising Hormone (LH) and Follicle Stimulating Hormone (FSH) – cooperate in the growth and release of an egg from a fluid-filled follicle within the ovary. While numerous follicles start growing each month, only one will become mature enough to ovulate. Ovulation (release of the egg from the ovary) is caused by an increase in LH about two weeks before menstruation starts.

On the other hand, during an IVF cycle, it is desirable for several eggs to mature at the same time with FSH injections, with a trigger injection, given to mature the developing eggs before collection. Hence the need for “pituitary suppression”.

There are different forms of IVF treatment: with pituitary suppression before starting stimulation (sometimes called long down regulation), and with pituitary suppression after the stimulation has started (sometimes called the antagonist cycle).

Your treating specialist will have selected the best cycle type for your individual needs.

We will provide you with detailed information about your specific treatment cycle, including the timeline and medications. You will also be shown how to administer your daily FSH injections and given instructions for any other medications you may need during your treatment cycle.

Step 2: Ovarian stimulation

This stage involves stimulating the ovaries with medication to promote the growth of follicles containing eggs. This is done via daily FSH injections for between 9 and 14 days. The FSH dose used is slightly higher than what a woman produces naturally – this is in the hope of stimulating the growth of multiple follicles.

The response of the ovaries is monitored with regular ultrasounds and blood tests. Most women learn to administer their own injections, or their partner may assist, and this helps to reduce the number of clinic visits.

Step 3: Trigger injection

The ovarian stimulation phase ends once ultrasounds show that the number and size of the follicles are adequate. The FSH injections and the GnRH agonist/antagonist (pituitary suppressant) are then stopped. Once as your fertility coordinator we have confirmed your procedure time with theatre, we will advise the exact time to administer your trigger injection. This is an injection of human chorionic gonadotrophin (hCG) to aid the egg’s final maturation and loosening it from the follicle wall.

Egg retrieval takes place on the second morning after the final injection (34-36 hours later). Your trigger injection timing is crucial, so ensure you write it down carefully with your instructions.

Step 4: Egg retrieval and semen collection

We will then perform the egg retrieval or egg “pick-up” while you are lightly sedated. This involves passing an ultrasound-guided probe, with a fine needle attached, through the vaginal canal and into each follicle in the ovary. The fluid in the follicle is then extracted into a test tube and studied under a microscope to look for eggs.

This procedure takes 5-10 minutes, depending on the number of follicles that have developed. After the egg retrieval, you will rest for one hour before going home. We recommend patients take the day off work and avoid any strenuous exercise.

On the day of your egg retrieval procedure, your partner will be asked to produce his semen sample. A men’s room is available at each clinic. If you and your partner have any concerns about collecting the sample, please discuss this with us before starting your cycle. It may be possible to produce the sample at home and drop it off at the clinic, or freeze one of your partner’s semen samples as a backup.

Step 5: Fertilization (insemination) and embryo development

Next, the sperm sample is washed and concentrated, then added to the eggs about four hours after egg retrieval. The sperm and eggs are placed in an incubator overnight and the eggs are examined the next day for signs of fertilization. Usually, 60 to 70 percent of eggs will fertilize if the sperm sample appears normal.

An embryologist from our team will contact you to discuss fertilization outcomes and any questions you may have. The fertilized eggs are then kept in the incubator for an additional 48 hours.

There’s a possibility of us suggesting Intracytoplasmic Sperm Injection (ICSI) as part of your treatment if the sperm quality is low (sperm count, morphology, or motility). ICSI is a specialized form of treatment for male infertility and involves injecting a single sperm directly into a single mature egg.

Step 6: Embryo transfer

Embryo transfer occurs two to five days after egg retrieval. The embryo chosen for transfer is inserted into a thin plastic catheter (a soft tube), which is passed through the cervix, into the uterus, and gently released. The process itself takes only a few minutes and is usually painless.

Generally, only one embryo is transferred, or two in exceptional cases – this will depend on your individual circumstances and embryo quality.

After the transfer, some of the remaining embryos may be suitable for vitrification (rapid freezing), and this option is available to all patients undergoing IVF treatment. Frozen embryos can be used in subsequent cycles if the first one is not successful. To be suitable for freezing, embryos must not show signs of abnormal/slow development or fragmentation (cell breakdown).

Step 7: Luteal phase

The luteal phase is the two-week period between the embryo transfer and the pregnancy test. During this time, it is important that you look after yourself and maintain good health and wellbeing. We will actively encourage you to limit strenuous activity for 24 hours after the transfer.

The progesterone medication you start taking after egg retrieval can sometimes cause tiredness, nausea, cramping, and bloating. You may choose to take an analgesic if you experience any discomfort. If you are concerned about any symptoms, connect with us immediately.

Vaginal spotting or bleeding may happen before you are due for your pregnancy test, but this does not always mean treatment was unsuccessful. You should continue using any treatment medications until a full period begins and/or until we have received your blood test results.

Step 8: Pregnancy test

Your pregnancy blood test is due about 14 days after the embryo transfer. A nurse from our team will call to give you the results and advise you on the next steps to take on your journey to having a child.

We recognize that the time between embryo transfer and your pregnancy test can be emotionally charged with anxiety and expectation. We understand this can be a difficult time and encourage you to call us for support if you are finding it hard to cope with the stress of waiting. Complete access to us as your professional counselors is also available as part of your IVF treatment.

We provide personalized, compassionate care and will support you at every step of your fertility journey.

For more information on the cost of treatment, potential risks, who IVF is recommended for, and to download a step-by-step infographic of the process, please visit our IVF treatment page.

  1. Thank you for sharing IVF process with us. I found it very interesting and useful for all the women’s in the world. Keep sharing such amazing blogs.

  2. Thank you for sharing IVF process with us. And how to maximize IVF treatment chances. I found it very interesting and useful for all the women’s in the world. Keep sharing such amazing blogs.

  3. I express my gratitude towards you for sharing information about IVF.Thanks for providing. Santhathi Fertility Centre offers the best infertility treatments in Bangalore. Our Fertility hospital in Bangalore has the best IVF specialists who are experts in IVF Treatments and offer you the right medical care for your gynec problems.For more info visit our official website at or Contact us at +91 94815 38470

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